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Fluorescent Primer(s) Creation for Nucleic Acid Detection and Amplification

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CDC researchers have developed technology that consists of a simple and inexpensive technique for creating fluorescent labeled primers for nucleic acid amplification. Fluorescent chemical-labeled probes and primers are extensively used in clinical and research laboratories for rapid, real-time detection and identification of microbes and genetic sequences. During nucleic acid amplification, the "UniFluor" primer is incorporated into newly synthesized double stranded DNA.

Inventor First Name: 
Jothikumar
Vincent
Inventor Last Name: 
Narayanan
Hill
E Number Only: 
E-252-2013
Inv Is lead: 
Pat Filing Date: 
2006-01-03
2006-01-03
2005-01-03
2006-01-03
Patent Authority: 
US
PCT
US
US
Patent Issue Date: 
2010-05-04
Patent Number: 
7,709,626
Provider Technology ID: 
2741
Patent Application: 
11/325,270
PCT/US2006/000175
60/641,303
12/267,869
LPM Zip: 
20892-9804
LPM Organization: 
NIH Office of Technology Transfer
LPM Phone: 
301-443-8518
LPM State: 
MD
  • Bioinformatics
  • Gene-based
  • Platform
Updated On: 
Monday, March 9, 2020
Related Patents: 
9,260,746
9,809,845
7,811,439
8,257,568
Related Patent Publish Date: 
Monday, August 6, 2012
Friday, November 21, 2008
Wednesday, May 19, 2010
Wednesday, November 21, 2007
Wednesday, February 4, 2015
Monday, February 15, 2016
Wednesday, August 3, 2005
Thursday, August 3, 2006
Thursday, October 7, 2010
Related Invention: 
E-273-2013-0
E-292-2013-0
E-458-2013-0
Related Patent Application: 

PCT/US2012/049784

PCT/US2008/084347

12/743,607

60/989,768

14/419,641

15/043,713

60/705,933

11/499,371

12/899,671

Related Patent Authority: 
PCT
PCT
US
US
US
US
US
US
US
LPM Last Name: 
Mitzelfelt
LPM FIrst Name: 
Jeremiah
Development Stage: 
Date Published: 
Monday, March 9, 2020
Development Status: 

In vitro data available

DTDT Classification: 
Devices/Instruments; Software; Mfg Technology/Methods; Research Tools & Material
Diagnostics
Methods
Research Tools and Materials
Instruments (e.g. spectrometers, microscopes)
Methods of Using Research Tools
Biologicals and Chemicals
Arthritis
Biodefense Related
Cancer
Cardiovascular Disease
Central Nervous System
Diabetes
Endocrine
Hyperlipidemia
Hypertension
Infectious Diseases (bacterial)
Infectious Diseases (fungal)
Infectious Diseases (viral)
Pain
Psychiatry
Zoonotic/Veterinary
Others
Biodefense
Diagnostic
Environmental Sampling, Testing
Monitoring (Public Health/Counterfeit Detection/Biological)
Research Tool
Bioinformatics
Gene-based
Platform
In vitro data available
DTDT Description: 
Devices/Instrumentation
Devices/Instrumentation - Diagnostics
Devices/Instrumentation - Diagnostics: Methods
Devices/Instrumentation - Research Tools and Materials
Devices/Instrumentation - Research Tools and Materials: Instruments
Devices/Instrumentation - Research Tools and Materials: Methods of Using Research Tools
Devices/Instrumentation - Research Tools and Materials: Biologicals and Chemicals
Arthritis
Biodefense Related
Cancer
Cardiovascular Disease
Central Nervous System
Diabetes
Endocrine
Hyperlipidemia
Hypertension
Infectious Diseases (bacterial)
Infectious Diseases (fungal)
Infectious Diseases (viral)
Pain
Psychiatry
Zoonotic/Veterinary
Others
Biodefense
Diagnostic
Applications - Environmental Sampling/Testing
Monitoring (Public Health/Counterfeit Detection/Biological)
Research Tool
Bioinformatics
Gene-based
Platform
In vitro data available
Collaboration Sought: 
Yes
Additional Patents: 
Several international patent applications pending or issued
Advantages: 
  • Simple to implement
  • Rapid, real-time detection
  • Used with standard laboratory equipment capable of monitoring fluorescence-intensity shifts
  • Cost-effective
  • Easily adapted for use in kits or arrays
Applications: 
  • Quantitative detection and/or amplification of specified nucleic acid sequences
  • Efficient fluorescence-labeling of oligonucleotides
  • Pyro-sequencing
  • Basic laboratory research
NIH Ref. No.: 
E-252-2013-0
IC: 
CDC
LPM Address: 
BG 5601FL RM 2G29 MSC 9804 5601 FISHERS LN
Inventor Lab URL: 
https://www.cdc.gov/media/spokesperson/
LPM City: 
Bethesda
Inventor IC: 
CDC
CDC
Institute or Center: 
Lead Inventor: 

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